Journal: Cancer Research

Article Title: Mitochondrial Transfer Rescues Respiration to Support De Novo Pyrimidine Biosynthesis and Tumor Progression

doi: 10.1158/0008-5472.CAN-24-0737

Figure Lengend Snippet: Absence of DHODH suppresses efficient tumor growth. A, Expression of DHODH in parental and DHODH KO cells (HCT116, MDA-MB-231, and PaTu 8902 cell lines) was assessed by Western blotting analysis. B –D , Routine and DHODH-dependent respiration was evaluated in parental and DHODH KO cells ( B and C ), as well as their proliferation (number of cells; D ) at 72 hours in the presence and absence of uridine in the media. E, NSG mice were grafted subcutaneously with parental and DHODH KO cells (10 6 per animal), and tumor volume was assessed using calipers ( n = 6 for each group). The experiments are representative of at least three independent repetitions ( n ≥ 3). Statistical analysis was performed using an unpaired t test with GraphPad Prism 8 software, considering differences with a P value of ≤ 0.05 as statistically significant. Images are representative of three independent experiments of three separate biological replicates. *, P value of 0.01 to 0.05; **, P value of 0.001 to 0.01.

Article Snippet: Human colorectal cancer HCT116, triple-negative breast cancer MDA-MB-231, and pancreatic cancer PaTu 8902 cells were purchased from the ATCC.

Techniques: Expressing, Western Blot, Software

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Inulin‐Butyrate Nanogel for Modulation of Gut Microbiome, Intestinal Barrier, and Regulatory T‐Cells in Colitis

doi: 10.1002/smll.202513252

Figure Lengend Snippet: IBN enhances intestinal barrier functions. (a‐c) Healthy or DSS‐colitis mice were orally administered on day ‐6. ‐4, ‐2, 0, 2, 4, 6 and 8 mice were orally administered with PBS, NaB (equivalent to their content in IBN), Inulin (equivalently to their content in IBN) or IBN (60 mg/dose). Colon tissues were excised and analyzed for the expression patterns (a) and mRNA levels (b, c) of ZO‐1 and occludin‐1. (d) mRNA expression levels of ZO‐1 in HCT‐116 cells treated with control medium or butyrate (0.5 mM) in the presence or absence of hydrogen peroxide (200 µ m ). Scale bars, 20 µm (a). Representative images from three slides are shown, obtained from n = 6 biologically independent animals across independent experiments. Data are presented as mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001, analyzed by one‐way ANOVA followed by LSD post hoc test.

Article Snippet: The HCT‐116 human colonic epithelial cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Control

Journal: Gut Microbes

Article Title: Fusobacterium sphaericum sp. nov., isolated from a human colon tumor adheres to colonic epithelial cells and induces IL-8 secretion

doi: 10.1080/19490976.2024.2442522

Figure Lengend Snippet: Fusobacterium sphaericum sp. nov. adheres to colonic epithelial cells and stimulates IL-8 secretion. Confocal laser scanning microscopy of colon cancer epithelial cell lines, HCT116 and HT-29, co-incubated with Fusobacterium nucleatum subsp. animalis ( Fna ) SB010 or F. sphaericum sp. nov. ( Fs ) SB021. Bacterial cell staining: FM4-64X (red) and DAPI (blue). Eukaryotic cell staining: DAPI (blue), actin (green). b–c) Quantitative levels of IL-8 secretion measured via the Single-Analyte ELISArray from HCT116 cells incubated alone or co-incubated with Escherichia coli TOP10, SB010, or SB021 at b) 4-hour and c) 24-hour time points. For c) additional co-incubations with dead SB021 and cell-free supernatant were performed. Statistical analysis performed via a one-way ANOVA. For a–c, all co-incubations were at a multiplicity of infection (MOI) of 100.

Article Snippet: We co-incubated HCT116 human colorectal carcinoma cell line (CCL-247; ATCC, Manassas, VA, USA) at low passage (<10) with F. sphaericum sp. nov. SB021 (OD 600 nm = 1.0) for 4 h followed by fixation with 1⁄2 strength Karnovsky’s fixative (2.5% glutaraldehyde and 2% paraformaldehyde in 0.1 M cacodylate buffer) and further sample preparation on the same day.

Techniques: Confocal Laser Scanning Microscopy, Incubation, Staining, Infection